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The Kjeldahl method's universality, precision and reproducibility have made it the internationally recognized method for estimating the protein content in foods and it is the standard method against which all other methods are judged. It is also used to assay soils, waste waters, fertilizers and other materials.
The NSI is the amount of Nitrogen in this filtered solution divided by the nitrogen in the initial sample, as measured by the Kjeldahl method. [ 3 ] The relevance of the NSI is based on the fact that proteins are the major biological source of Nitrogen: for various types of protein, there are empirical formulas which correlate the nitrogen ...
Johan Gustav Christoffer Thorsager Kjeldahl (Danish pronunciation: [joˈhæn ˈkʰelˌtɛˀl] 16 August 1849 – 18 July 1900), was a Danish chemist who developed a method for determining the amount of nitrogen in certain organic compounds using a laboratory technique which was named the Kjeldahl method after him.
There are some more methods like Liebig method / Duma's method / Kjeldahl's method and Carius method for estimation of organic compounds. For example, quantitative analysis performed by mass spectrometry on biological samples can determine, by the relative abundance ratio of specific proteins, indications of certain diseases, like cancer.
protein by analysis of total nitrogen, either by Dumas or Kjeldahl methods; total fat, traditionally by a solvent extraction, but often now by secondary methods such as NMR; crude ash (total inorganic matter) by combustion at 550 °C; estimated dietary fibre by various AOAC methods such as 985.29
Protein methods are the techniques used to study proteins. There are experimental methods for studying proteins (e.g., for detecting proteins, for isolating and purifying proteins, and for characterizing the structure and function of proteins, [1] often requiring that the protein first be purified). Computational methods typically use computer ...
An alternative method for label free protein quantification in clear liquid is cuvette-based SPR technique, that simultaneously measures the refractive index ranging 1.0 to 1.6 nD and concentration of the protein ranging from 0.5 μL to 2 mL in volume. This system consists of the calibrated optical filter with very high angular resolution and ...
Used to detect the presence of a ligand (commonly a protein) in a liquid sample using antibodies directed against the protein to be measure: Biochemistry, Molecular biology: Gene knockout: Used to make one of an organism's genes inoperative ("knocked out" of the organism) Molecular biology, Genetics: Immunostaining