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Diagram of a simple microscope. There are two basic types of optical microscopes: simple microscopes and compound microscopes. A simple microscope uses the optical power of a single lens or group of lenses for magnification. A compound microscope uses a system of lenses (one set enlarging the image produced by another) to achieve a much higher ...
Schematic of a fluorescence microscope The majority of fluorescence microscopes, especially those used in the life sciences , are of the epifluorescence design shown in the diagram. Light of the excitation wavelength illuminates the specimen through the objective lens.
Köhler illumination is a method of specimen illumination used for transmitted and reflected light (trans- and epi-illuminated) optical microscopy.Köhler illumination acts to generate an even illumination of the sample and ensures that an image of the illumination source (for example a halogen lamp filament) is not visible in the resulting image.
Schematic representation of the energy levels (Jabłoński diagrams) of the fluorescence process, example of a fluorescent dye that emits light at 460 nm. One (purple, 1PEF), two (light red, 2PEF) or three (dark red, 3PEF) photons are absorbed to emit a photon of fluorescence (turquoise). Optical response from a point source.
Diagram illustrating the light path through a dark-field microscope. The steps are illustrated in the figure where an inverted microscope is used. Light enters the microscope for illumination of the sample. A specially sized disc, the patch stop (see figure), blocks some light from the light source, leaving an outer ring of illumination. A wide ...
Figure 3: Schematic layout of an interference microscope with Mirau objective. To visualize microscopic structures, it is necessary to combine an interferometer with the optics of a microscope. Such an arrangement is shown in Figure 3. This setup is similar to a standard optical microscope.
Schematic ray diagram illustrating the optical reciprocity between TEM (left) and STEM (right). The convergence angle in TEM, , becomes the collection angle in STEM, . Image inspired by Hren et al. [22] Fundamentally, TEM and STEM are linked via Helmholtz reciprocity. A STEM is a TEM in which the electron source and observation point have been ...
Schematic of STEM mode An ultrahigh-vacuum STEM equipped with a 3rd-order spherical aberration corrector Inside the aberration corrector (hexapole-hexapole type) A scanning transmission electron microscope (STEM) is a type of transmission electron microscope (TEM). Pronunciation is [stɛm] or [ɛsti:i:ɛm].