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A stool test is a medical diagnostic technique that involves the collection and analysis of fecal matter. Microbial analysis (culturing), microscopy and chemical ...
Human feces photographed in a toilet, shortly after defecation.. Human feces (American English) or faeces (British English), commonly and in medical literature more often called stool, [1] are the solid or semisolid remains of food that could not be digested or absorbed in the small intestine of humans, but has been further broken down by bacteria in the large intestine.
The fecal coliform assay is intended to be an indicator of fecal contamination; more specifically of E. coli which is an indicator microorganism for other pathogens that may be present in feces. Presence of fecal coliforms in water may not be directly harmful, and do not necessarily indicate the presence of feces. [1]
Escherichia coli (E. coli) can be distinguished from most other coliforms by its ability to ferment lactose at 44 °C in the fecal coliform test, and by its growth and color reaction on certain types of culture media.
Stool is a common term in reference to human feces. For example, in medicine, to diagnose the presence or absence of a medical condition, a stool sample sometimes is requested for testing purposes. [33] The term bowel movement(s) (with each movement a defecation event) is also common in health care.
It requires a minimum of 2 days and maximum of several weeks to culture gastrointestinal pathogens. The sensitivity (true positive) and specificity (true negative) rates for stool culture vary by pathogen, although a number of human pathogens can not be cultured. For culture-positive samples, antimicrobial resistance testing takes an additional ...
Furthermore, the term culture is more generally used informally to refer to "selectively growing" a specific kind of microorganism in the lab. It is often essential to isolate a pure culture of microorganisms. A pure (or axenic) culture is a population of cells or multicellular organisms growing in the absence of other species or types.
The presence of some coliforms indicate fecal contamination. The term "IMViC" is an acronym for each of these tests. "I" is for indole test; "M" is for methyl red test; "V" is for Voges-Proskauer test, and "C" is for citrate test. The lower case "i" is merely for "in" as the Citrate test requires coliform samples to be placed "in Citrate".