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  2. Flow cytometry - Wikipedia

    en.wikipedia.org/wiki/Flow_cytometry

    Flow cytometry (FC) is a technique used to detect and measure the physical and chemical characteristics of a population of cells or particles. [1] [2] [3] [4]In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument.

  3. Cell cycle analysis - Wikipedia

    en.wikipedia.org/wiki/Cell_cycle_analysis

    Cell cycle analysis by DNA content measurement is a method that most frequently employs flow cytometry to distinguish cells in different phases of the cell cycle.Before analysis, the cells are usually permeabilised and treated with a fluorescent dye that stains DNA quantitatively, such as propidium iodide (PI) or 4,6-diamidino-2-phenylindole (DAPI).

  4. Hematology analyzer - Wikipedia

    en.wikipedia.org/wiki/Hematology_analyzer

    This type of hematology analyzer utilizes both Coulter's principle and flow cytometry to determine the granularity, diameter, and inner complexity of the cells. Using hydrodynamic focusing, the cells are sent through an aperture one cell at a time.

  5. Cell counting - Wikipedia

    en.wikipedia.org/wiki/Cell_counting

    Flow cytometry is by far the most sophisticated and expensive method for cell counting. In a flow cytometer the cells flow in a narrow stream in front of a laser beam. The beam hits them one by one, and a light detector picks up the light that is reflected from the cells.

  6. Cytometry - Wikipedia

    en.wikipedia.org/wiki/Cytometry

    Cytometers are the instruments which count the blood cells in the common blood test.. Cytometry is the measurement of number and characteristics of cells.Variables that can be measured by cytometric methods include cell size, cell count, cell morphology (shape and structure), cell cycle phase, DNA content, and the existence or absence of specific proteins on the cell surface or in the ...

  7. Cell sorting - Wikipedia

    en.wikipedia.org/wiki/Cell_sorting

    Flow cytometry cell-sorting yields very high specificity according to one or several surface markers, but one limitation is constituted by the number of cells that can be processed during a work day. For this reason pre-enrichment of the population of interest by immunomagnetic cell sorting is often considered, especially when the target cells ...

  8. Tetramer assay - Wikipedia

    en.wikipedia.org/wiki/Tetramer_assay

    The tetramers are labeled with a fluorophore, allowing tetramer-bound T-cells to be analyzed with flow cytometry. [4] Quantification and sorting of T-cells by flow cytometry enables researchers to investigate immune response to viral infection and vaccine administration as well as functionality of antigen-specific T-cells. [5]

  9. CyTOF - Wikipedia

    en.wikipedia.org/wiki/CyTOF

    This file contains the total ion counts for each channel for every cell arranged in a matrix and is the same file generated during flow cytometry. [5] Manual gating of this data can be performed as is done for flow cytometry and most of the tools available for flow cytometry analysis have been ported to CyTOF (See flow cytometry bioinformatics ...

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