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In another approach to studying photosynthesis, light-absorbing pigments such as chlorophyll can be extracted from chloroplasts. Like so many important biological systems in the cell, the photosynthetic system is ordered and compartmentalized in a system of membranes. [9] Isolated chloroplasts from spinach leaves, viewed under light microscope
Another redox protein, isolated from spinach chloroplasts, was termed "chloroplast ferredoxin". [3] The chloroplast ferredoxin is involved in both cyclic and non-cyclic photophosphorylation reactions of photosynthesis. In non-cyclic photophosphorylation, ferredoxin is the last electron acceptor thus reducing the enzyme NADP + reductase.
Liu et al. (2004) Crystal structure of spinach major light-harvesting complex at 2.72A° resolution. Nature 428: 287–292. Lokstein (1994)The role of light-harvesting complex II energy dissipation: an in-vivo fluorescence in excess excitation study on the origin of high-energy quenching. Journal of Photochemistry and Photobiology 26: 175-184
The following is a breakdown of the energetics of the photosynthesis process from Photosynthesis by Hall and Rao: [6]. Starting with the solar spectrum falling on a leaf, 47% lost due to photons outside the 400–700 nm active range (chlorophyll uses photons between 400 and 700 nm, extracting the energy of one 700 nm photon from each one)
In parallel, plant physiologists studied leaf gas exchanges using the new method of infrared gas analysis and a leaf chamber where the net photosynthetic rates ranged from 10 to 13 μmol CO 2 ·m −2 ·s −1, with the conclusion that all terrestrial plants have the same photosynthetic capacities, that are light saturated at less than 50% of ...
As DCPIP is reduced and becomes colorless, the resultant increase in light transmittance can be measured using a spectrophotometer. DCPIP can also be used as an indicator for vitamin C . [ 1 ] [ 2 ] If vitamin C, which is a good reducing agent, is present, the blue dye, which turns pink in acid conditions, is reduced to a colorless compound by ...
The δ 13 C of C3 plants depends on the relationship between stomatal conductance and photosynthetic rate, which is a good proxy of water use efficiency in the leaf. [19] C3 plants with high water-use efficiency tend to be less fractionated in 13 C (i.e., δ 13 C is relatively less negative) compared to C3 plants with low water-use efficiency. [19]
A photosynthesis system analysing the photosynthetic rate of a maize leaf. Photosynthesis systems are electronic scientific instruments designed for non-destructive measurement of photosynthetic rates in the field. Photosynthesis systems are commonly used in agronomic and environmental research, as well as studies of the global carbon cycle.