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Methods in Molecular Biology is a book series published by Humana Press (an imprint of Springer Science+Business Media) that covers molecular biology research methods and protocols. The book series was introduced by series editor John M. Walker in 1983 and provides step-by-step instructions for carrying out experiments in a research lab. [1]
Springer Protocols contained more than 33,000 protocols, most of which were derived from the book series Methods in Molecular Biology, published under the Humana Press imprint. That book series, edited by John M. Walker since 1984, contains more than 1,100 volumes and has spawned several related book series.
Biochemistry, Molecular biology: Gene knockout: Used to make one of an organism's genes inoperative ("knocked out" of the organism) Molecular biology, Genetics: Immunostaining: Used of an antibody-based method to detect a specific protein in a sample: Molecular biology, Biochemistry: Intracellular recording: Used to measure the voltage across a ...
Molecular biology is the study of the molecular underpinnings of the biological phenomena, focusing on molecular synthesis, modification, mechanisms and interactions. Biochemistry is the study of the chemical substances and vital processes occurring in living organisms .
The methods used for clinical viral metagenomics are not standardized, but guidelines have been published by the European Society for Clinical Virology. [ 32 ] [ 33 ] A mixture of different sequencing platforms are used for clinical viral metagenomics, the most common being instruments from Illumina and Oxford Nanopore Technologies .
Protein purification is a critical process in molecular biology and biochemistry, aimed at isolating a specific protein from a complex mixture, such as cell lysates or tissue extracts. [9] The goal is to obtain the protein in a pure form that retains its biological activity for further study, including functional assays, structural analysis, or ...
This method is an extension of bisulfite sequencing, which is the gold standard for determining DNA methylation. [2] NOMe-seq relies on the methyltransferase M.CviPl, which methylates cytosines in GpC dinucleotides unbound by nucleosomes or other proteins , creating a nucleosome footprint.
DamID is a method developed in 2000 by Steven Henikoff for identifying parts of the genome proximal to a chromatin protein of interest. DamID relies on a DNA methyltransferase fusion to the chromatin protein to nonnaturally methylate DNA, which can then be subsequently sequenced to reveal genome methylation sites near the protein. [ 4 ]