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Capillary electrophoresis (CE) is a family of electrokinetic separation methods performed in submillimeter diameter capillaries and in micro- and nanofluidic channels.Very often, CE refers to capillary zone electrophoresis (CZE), but other electrophoretic techniques including capillary gel electrophoresis (CGE), capillary isoelectric focusing (CIEF), capillary isotachophoresis and micellar ...
The original interface between capillary zone electrophoresis and mass spectrometry was developed in 1987 [9] by Richard D. Smith and coworkers at Pacific Northwest National Laboratory, and who also later were involved in development of interfaces with other CE variants, including capillary isotachophoresis and capillary isoelectric focusing.
Pittcon Editors’ Awards honoured the best new products on show at the Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, or Pittcon, [1] for 20 years from 1996 having been established by Dr Gordon Wilkinson, managing editor of Analytical Instrument Industry Report (later Instrumenta).
[1] [2] Capillary electrochromatography is a combination of two analytical techniques, high-performance liquid chromatography and capillary electrophoresis. Capillary electrophoresis aims to separate analytes on the basis of their mass-to-charge ratio by passing a high voltage across ends of a capillary tube , which is filled with the analyte.
SCIEX is a manufacturer of mass spectrometry instrumentation used in biomedical and environmental applications. Originally started by scientists from the University of Toronto Institute for Aerospace Studies, it is now part of Danaher Corporation with the SCIExe R&D division still located in Toronto, Canada.
More detailed description and several applications of KCE methods (measuring equilibrium and rate constants of molecular interactions, quantitative affinity analysis of proteins, thermochemistry of protein–ligand interactions, selection of aptamers, determination of temperature inside a capillary) can be found in a PDF presentation: KCE is a conceptual platform for kinetic homogeneous ...
It is a modification of capillary electrophoresis (CE), extending its functionality to neutral analytes, [1] where the samples are separated by differential partitioning between micelles (pseudo-stationary phase) and a surrounding aqueous buffer solution (mobile phase). [2]
The apparatus consists of a vial with a conical bottom, a grounded platinum electrode, a capillary to inject the aqueous solution, and an adjustable gold anode with a circular bottom that contacts the entire organic phase. EE is also often performed in a capillary electrophoresis capillary. This is referred to as capillary electroextraction, or ...
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