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  2. DNA extraction - Wikipedia

    en.wikipedia.org/wiki/DNA_extraction

    DNA extraction is the process of isolating DNA from the cells of an organism isolated from a sample, typically a biological sample such as blood, saliva, or tissue. It involves breaking open the cells, removing proteins and other contaminants, and purifying the DNA so that it is free of other cellular components.

  3. DNA separation by silica adsorption - Wikipedia

    en.wikipedia.org/wiki/DNA_separation_by_silica...

    In order to separate DNA through silica adsorption, a sample is first lysed, releasing proteins, DNA, phospholipids, etc. from the cells. The remaining tissue is discarded. The supernatant containing the DNA is then exposed to silica in a solution with high ionic strength. The highest DNA adsorption efficiencies occur in the presence of buffer ...

  4. Spin column-based nucleic acid purification - Wikipedia

    en.wikipedia.org/wiki/Spin_column-based_nucleic...

    Silica in a spin column with water and with DNA sample in chaotropic buffer. Spin column-based nucleic acid purification is a solid phase extraction method to quickly purify nucleic acids. This method relies on the fact that nucleic acid will bind to the solid phase of silica under certain conditions.

  5. Gel extraction - Wikipedia

    en.wikipedia.org/wiki/Gel_extraction

    Gel extraction kits are available from several major biotech manufacturers for a final cost of approximately 1–2 US$ per sample. Protocols included in these kits generally call for the dissolution of the gel-slice in 3 volumes of chaotropic agent at 50 °C, followed by application of the solution to a spin-column (the DNA remains in the column), a 70% ethanol wash (the DNA remains in the ...

  6. Boom method - Wikipedia

    en.wikipedia.org/wiki/Boom_method

    Boom method (aka Boom nucleic acid extraction method) is a solid phase extraction method for isolating nucleic acid from a biological sample. This method is characterized by "absorbing the nucleic acids (NA) to the silica beads".

  7. Acid guanidinium thiocyanate-phenol-chloroform extraction

    en.wikipedia.org/wiki/Acid_guanidinium...

    RNA partitions in the aqueous phase, while proteins and DNA partition into the organic/interphase (left). The RNA is then precipitated in an alcohol (right). Acid guanidinium thiocyanate-phenol-chloroform extraction (abbreviated AGPC) is a liquid–liquid extraction technique in biochemistry and molecular biology.

  8. DNA profiling - Wikipedia

    en.wikipedia.org/wiki/DNA_profiling

    The most common methods of DNA extraction include organic extraction (also called phenol–chloroform extraction), [17] Chelex extraction, and solid-phase extraction. Differential extraction is a modified version of extraction in which DNA from two different types of cells can be separated from each other before being purified from the solution ...

  9. Chelex 100 - Wikipedia

    en.wikipedia.org/wiki/Chelex_100

    Chelex resin is often used for DNA extraction in preparation for polymerase chain reaction by binding to cations including Mg 2+, which is an essential cofactor for DNases. Chelex protects the sample from DNases that might remain active after the boiling and could subsequently degrade the DNA, rendering it unsuitable for PCR.