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Ammonium sulfate is an inorganic salt with a high solubility that disassociates into ammonium (NH + 4) and sulfate (SO 2− 4) in aqueous solutions. [1] Ammonium sulfate is especially useful as a precipitant because it is highly soluble, stabilizes protein structure, has a relatively low density, is readily available, and is relatively inexpensive.
The precipitation method is the one used for the determination of the amount of calcium in water. Using this method, an excess of oxalic acid, H 2 C 2 O 4, is added to a measured, known volume of water. By adding a reagent, here ammonium oxalate, the calcium will precipitate as calcium oxalate. The proper reagent, when added to aqueous solution ...
Ammonium sulfate precipitation is a common method for protein purification by precipitation. As the ionic strength of a solution increases, the solubility of proteins in that solution decreases. Being extremely soluble in water, ammonium sulfate can "salt out" (precipitate) proteins from aqueous solutions.
The following chart shows the solubility of various ionic compounds in water at 1 atm pressure and room temperature (approx. 25 °C, 298.15 K). "Soluble" means the ionic compound doesn't precipitate, while "slightly soluble" and "insoluble" mean that a solid will precipitate; "slightly soluble" compounds like calcium sulfate may require heat to precipitate.
This depth is converted to a flow rate according to a theoretical formula of the form = where is the flow rate, is a constant, is the water level, and is an exponent which varies with the device used; or it is converted according to empirically derived level/flow data points (a "flow curve"). The flow rate can then be integrated over time into ...
Salting out (also known as salt-induced precipitation, salt fractionation, anti-solvent crystallization, precipitation crystallization, or drowning out) [1] is a purification technique that utilizes the reduced solubility of certain molecules in a solution of very high ionic strength.
By contrast, later salts in the series increase the solubility of nonpolar molecules ("salting in") and decrease the order in water; in effect, they weaken the hydrophobic effect. [14] [15] The "salting out" effect is commonly exploited in protein purification through the use of ammonium sulfate precipitation. [16]
The rate of flow is typically expressed in units of cubic meters per second (m³/s) or cubic feet per second (cfs). Hydrographs often relate changes of precipitation to changes in discharge over time. [3] The term can also refer to a graph showing the volume of water reaching a particular outfall, or location in a sewerage network. Graphs are ...
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