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An inoculation loop (also called a smear loop, inoculation wand or microstreaker) is a simple tool used mainly by microbiologists to pick up and transfer a small sample of microorganisms called inoculum from a microbial culture, e.g. for streaking on a culture plate. [1] [2] This process is called inoculation.
Thin smears are similar to usual blood films and allow species identification, because the parasite's appearance is best preserved in this preparation. Thick smears allow the microscopist to screen a larger volume of blood and are about eleven times more sensitive than the thin film, so picking up low levels of infection is easier on the thick ...
The cytocentrifugation process can cause cells to appear distorted. Cells located at the centre of the smear may look compressed compared to cells at the periphery. Cell nuclei may develop artifactual clefts, lobes, or holes, [4] and the cytoplasm may appear vacuolated or develop irregular projections. Cytoplasmic granules may be pushed to the ...
Microtechnique is an aggregate of methods used to prepare micro-objects for studying. [1] It is currently being employed in many fields in life science. Two well-known branches of microtechnique are botanical (plant) microtechnique and zoological (animal) microtechnique.
Preparation Application Result 1. Simple (Monochrome) Smear stain with single dye . e.g. Methylene blue, Safranin°≤×←→ etc. Used to highlight microbes and illustrate cellular shapes and arrangements Organisms are stained in the color of applied stain 2. Negative (Relief) Smear mixed with Nigrosin and spread into thin film
For example, a common application of cytopathology is the Pap smear, a screening tool used to detect precancerous cervical lesions that may lead to cervical cancer. Cytopathologic tests are sometimes called smear tests because the samples may be smeared across a glass microscope slide [ 4 ] for subsequent staining and microscopic examination.
Applying a primary stain (crystal violet) to a heat-fixed smear of a bacterial culture. Heat fixation kills some bacteria but is mostly used to affix the bacteria to the slide so that they do not rinse out during the staining procedure. The addition of iodine, which binds to crystal violet and traps it in the cell
When looking at the smears for TB, it is stained using an acid-fast stain. These acid-fast organisms like Mycobacterium contain large amounts of lipid substances within their cell walls called mycolic acids. These acids resist staining by ordinary methods such as a Gram stain. [9] It can also be used to stain a few other bacteria, such as Nocardia.