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Recently a web-based database of human and mouse housekeeping genes and reference genes/transcripts, named Housekeeping and Reference Transcript Atlas (HRT Atlas), was developed to offer updated list of housekeeping genes and reliable candidate reference genes/transcripts for RT-qPCR data normalization. [1]
HRT Atlas allows searching of a complete list of reliable candidate reference genes and transcripts for RT-qPCR normalization in more than 120 human and mouse tissues or cell types. The database also offers some empirically validated primers and predicted modifiers (disease and small molecules) of the expression of these reference genes.
These genes are often selected from housekeeping genes as their functions related to basic cellular survival normally imply constitutive gene expression. [ 5 ] [ 6 ] This enables researchers to report a ratio for the expression of the genes of interest divided by the expression of the selected normalizer, thereby allowing comparison of the ...
Housekeeping and Reference Transcript Atlas (HRT Atlas) [27] web-based tool for searching cell specific candidate reference genes/transcripts suitable for qPCR experiment normalization. HRT Atlas also describes a complete list of human and mouse housekeeping genes and transcripts
Actin beta is often used in Western blotting as a loading control, to normalize total protein amounts and check for eventual protein degradation in the samples.Its transcript is also commonly used as a housekeeping gene standard in qPCR.
However, she later had a “horrible experience,” possibly due to “this gene, GDF15, which makes you extremely prone to nausea,” she said on Howard Stern’s SiriusXM show last month. “I ...
SINCE THE LATE 2010's, the carnivore diet has been all over social media. And it doesn't seem to be going anywhere any time soon. As its name indicates, the carnivore diet is a diet that only ...
Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells, it is considered a housekeeping gene. For this reason, GAPDH is commonly used by biological researchers as a loading control for western blot and as a control for qPCR.
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