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Mouse Embryonic Fibroblasts (MEFs) are a type of fibroblast prepared from mouse embryo. MEFs show a spindle shape when cultured in vitro, a typical feature of fibroblasts. The MEF is a limited cell line. After several transmissions, MEFs will senesce and finally die off.
EMAGE (e-Mouse Atlas of Gene Expression [note 1]) is an online biological database of gene expression data in the developing mouse (Mus musculus) embryo. [1] [2] [3] The data held in EMAGE is spatially annotated to a framework of 3D mouse embryo models produced by EMAP (e-Mouse Atlas Project).
The primary mouse embryonic fibroblast cells were transferred (the "T") every 3 days (the first "3"), and inoculated at the rigid density of 3 × 10 5 cells per 20 cm 2 dish (the second "3") continuously. [2] The spontaneously immortalized cells with stable growth rate were established after 20 to 30 generations in culture, and then named '3T3 ...
The "fate" of each cell or group of cells is mapped onto the embryo, showing which parts of the embryo will develop into which tissue. When carried out at single-cell resolution, this process is called cell lineage tracing. It is also used to trace the development of tumors. Fate mapping and cell lineage are similar methods for tracing the ...
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Diagram of stages of embryo development to a larval and adult stage. In developmental biology, animal embryonic development, also known as animal embryogenesis, is the developmental stage of an animal embryo. Embryonic development starts with the fertilization of an egg cell (ovum) by a sperm cell (spermatozoon). [1]
The anterior/posterior axis in the mouse embryo is specified by two signaling centers. In the mouse embryo, the egg forms a cylinder with the epiblast forming a cup at the distal end of that cylinder. The epiblast is surrounded by the visceral endoderm, the equivalent of the hypoblast of humans and chicks.
Software architecture is about making fundamental structural choices that are costly to change once implemented. Software architecture choices include specific structural options from possibilities in the design of the software. There are two fundamental laws in software architecture: [3] [4] Everything is a trade-off "Why is more important ...