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The glucose oxidase enzyme (GOx or GOD) also known as notatin (EC number 1.1.3.4) is an oxidoreductase that catalyses the oxidation of glucose to hydrogen peroxide and D-glucono-δ-lactone. This enzyme is produced by certain species of fungi and insects and displays antibacterial activity when oxygen and glucose are present.
The Trinder glucose activity test is a diagnostic test used in medicine to determine the presence of glucose or glucose oxidase. The test employs the Trinder reagent , and is a colour change test resulting from the Trinder reaction .
A colorless solid, it is an oxidized derivative of glucose. It is typically produced by the aerobic oxidation of glucose in the presence of the enzyme glucose oxidase. The conversion cogenerates hydrogen peroxide, which is often the key product of the enzyme: C 6 H 12 O 6 + O 2 → C 6 H 10 O 6 + H 2 O 2
He eventually found that these bacteria could only thrive adjacent to Aspergillus niger if glucose was not present. He eventually isolated the factor that caused the curious effect. The factor was glucose oxidase. In the presence of glucose, the glucose oxidase produced hydrogen peroxide, which killed off the bacteria. [1] [2] [3]
More specific than glucose oxidase method due to G-6PO 4, which inhibits interfering substances except when sample is hemolyzed. Clinical correlation The fasting blood glucose level, which is measured after a fast of 8 hours, is the most commonly used indication of overall glucose homeostasis, largely because disturbing events such as food ...
American Society for Microbiology, Oxidase Test Protocol. 2013. ASM MicrobeLibrary, 1–9. Cheng W J, Lin C W, Wu T G, Su C S, Hsieh M S. 2013. Calibration of glucose oxidase-based test strips for capillary blood measurement with oxygen saturated venous blood samples. Clinica Chimica Acta. 415, 152–157.
An important example is EC 7.1.1.9 cytochrome c oxidase, the key enzyme that allows the body to employ oxygen in the generation of energy and the final component of the electron transfer chain. Other examples are: EC 1.1.3.4 Glucose oxidase; EC 1.4.3.4 Monoamine oxidase; EC 1.14.-.- Cytochrome P450 oxidase; EC 1.6.3.1 NADPH oxidase
The Clark oxygen electrode laid the basis for the first glucose biosensor (in fact the first biosensor of any type), invented by Clark and Lyons in 1962. [6] This sensor used a single Clark oxygen electrode coupled with a counter-electrode. As with the Clark electrode, a permselective membrane covers the Pt electrode.