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A Ziehl–Neelsen stain is an acid-fast stain used to stain species of Mycobacterium tuberculosis that do not stain with the standard laboratory staining procedures such as Gram staining. This stain is performed through the use of both red coloured carbol fuchsin that stains the bacteria and a counter stain such as methylene blue.
In hemocytometry, Türk's solution (or Türk's fluid) is a hematological stain (either crystal violet or aqueous methylene blue) prepared in 99% acetic acid (glacial) [1] and distilled water. The solution destroys the red blood cells and platelets within a blood sample (acetic acid being the main lyzing agent ), and stains the nuclei of the ...
Crystal violet is also used as a tissue stain in the preparation of light microscopy sections. [15] In laboratory, solutions containing crystal violet and formalin are often used to simultaneously fix and stain cells grown in tissue culture to preserve them and make them easily
Gram-positive bacteria take up the crystal violet stain used in the test, and then appear to be purple-coloured when seen through an optical microscope. This is because the thick layer of peptidoglycan in the bacterial cell wall retains the stain after it is washed away from the rest of the sample, in the decolorization stage of the test.
Gram staining differentiates bacteria by the chemical and physical properties of their cell walls. Gram-positive cells have a thick layer of peptidoglycan in the cell wall that retains the primary stain, crystal violet. Gram-negative cells have a thinner peptidoglycan layer that allows the crystal violet to wash out on addition of ethanol.
Cell counting is any of various methods for the counting or similar quantification of cells in the life sciences, including medical diagnosis and treatment. It is an important subset of cytometry , with applications in research and clinical practice.
Cresyl violet stained partial brain section of a Macaque. It is used in biology and medicine as a histological stain. Cresyl violet is an effective and reliable stain used for light microscopy sections. Initially, tissue sections are "defatted" by passing through graded dilutions of ethanol. Then, rehydrated by passing back through decreasing ...
Due to its short staining time, Diff-Quik stain is often used for initial screening of cytopathology specimens. This staining technique allows the cytotechnologist or pathologist to quickly assess the adequacy of the specimen, identify possible neoplastic or inflammatory changes, and decide whether or not additional staining is required. [4] [9 ...