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Alternatively, if amplicon sizes overlap, the different amplicons may be differentiated and visualised using primers that have been dyed with different colour fluorescent dyes. Commercial multiplexing kits for PCR are available and used by many forensic laboratories to amplify degraded DNA samples.
In the biological sciences, a multiplex assay is a type of immunoassay that uses magnetic beads to simultaneously measure multiple analytes in a single experiment. [1] A multiplex assay is a derivative of an ELISA using beads for binding the capture antibody.
A multiplexing technique may be further extended into a multiple access method or channel access method, for example, TDM into time-division multiple access (TDMA) and statistical multiplexing into carrier-sense multiple access (CSMA). A multiple-access method makes it possible for several transmitters connected to the same physical medium to ...
In analytical chemistry, a tandem mass tag (TMT) is a chemical label that facilitates sample multiplexing in mass spectrometry (MS)-based quantification and identification of biological macromolecules such as proteins, peptides and nucleic acids.
Example of exon deletions detected by Multiplex ligation-dependent probe amplification in a Duchenne muscular dystrophy patient. MLPA quantifies the presence of particular sequences in a sample of DNA, using a specially designed probe pair for each target sequence of interest. The process consists of multiple steps: [3]
Multiplexing and multiple access may refer to: Multiplexing , a method by which multiple analog or digital signals are combined into one signal over a shared medium Multiple access , allows several terminals connected to the same transmission medium to transmit over a shared medium.
It is a multidisciplinary field that involves molecular analysis, molecular biology, and microelectronics. [1] It has practical applications in the design of systems that process low volumes of fluids to achieve multiplexing, automation, and high-throughput screening.
The amplification reaction initiates when multiple primer hexamers anneal to the template. When DNA synthesis proceeds to the next starting site, the polymerase displaces the newly produced DNA strand and continues its strand elongation.