Search results
Results from the WOW.Com Content Network
DMEM (Dulbecco's modified Eagle's medium) was originally suggested as Eagle's medium with a 'fourfold concentration of amino acids and vitamins' by Renato Dulbecco and G. Freeman published in 1959. [5] The commercial versions of this medium have additional modifications, see an example in the table below. [6]
Phenol red exists as a red crystal that is stable in air. Its solubility is 0.77 grams per liter (g/L) in water and 2.9 g/L in ethanol. [1] It is a weak acid with pK a = 8.00 at 20 °C (68 °F). A solution of phenol red is used as a pH indicator, often in cell culture.
Tissue culture flasks. RPMI 1640, simply known as RPMI medium, is a cell culture medium commonly used to culture mammalian cells. [1] RPMI 1640 was developed by George E. Moore, Robert E. Gerner, and H. Addison Franklin in 1966 at Roswell Park Comprehensive Cancer Center (formerly known as Roswell Park Memorial Institute), from where it derives its name. [2]
A bottle of DMEM cell culture medium. Aside from temperature and gas mixture, the most commonly varied factor in culture systems is the cell growth medium. Recipes for growth media can vary in pH, glucose concentration, growth factors, and the presence of other nutrients.
Human MCF-7 are cultivated in Dulbecco’s modified Eagle’s medium (DMEM) with fetal bovine serum (FBS) and phenol red as buffer tracer (culture medium), at 37 °C, in an atmosphere of 5% CO₂ and 95% air under saturating humidity.
For the Israeli hostages freed Saturday, the suffering did not end when Hamas militants paraded their frail and gaunt figures on a stage in Gaza ahead of their release to the Red Cross.
For example, if the concentration of the conjugate base is 10 times greater than the concentration of the acid, their ratio is 10:1, and consequently the pH is pK a + 1 or pK b + 1. Conversely, if a 10-fold excess of the acid occurs with respect to the base, the ratio is 1:10 and the pH is p K a − 1 or p K b − 1.
Tom Anderson, who co-founded MySpace in August 2003 at just 32 years old, was recently spotted at Costa Mesa Country Club in Southern California. “Tom is living all our dreams,” another ...