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Plant callus (plural calluses or calli) is a growing mass of unorganized plant parenchyma cells. In living plants, callus cells are those cells that cover a plant wound. In biological research and biotechnology callus formation is induced from plant tissue samples (explants) after surface sterilization and plating onto tissue culture medium in vitro (in a closed culture vessel such as a Petri ...
Hence a plant stem cell originated from cambium is an immortal cell while that from callus is a temporarily dediffertiated cell obtained from stimulating the somatic cell. Furthermore, the ability to differentiate and proliferate is different that differences between plant stem cell and callus are prevalent in culture and research.
While Manning's research was about plants, it helped establish the foundation for our modern-day understanding of dedifferentiation and cell plasticity. Just as plant cells respond to injury by undergoing callus formation via dedifferentiation, some animal models dedifferentiate their cells to form blastema, which are analogous to plant ...
In addition to growth by cell division, a plant may grow through cell elongation. This occurs when individual cells or groups of cells grow longer. Not all plant cells grow to the same length. When cells on one side of a stem grow longer and faster than cells on the other side, the stem bends to the side of the slower growing cells as a result.
Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues, or organs under sterile conditions on a nutrient culture medium of known composition. It is widely used to produce clones of a plant in a method known as micropropagation .
Somatic embryos are formed from plant cells that are not normally involved in the development of embryos, i.e. ordinary plant tissue. No endosperm or seed coat is formed around a somatic embryo. Cells derived from competent source tissue are cultured to form an undifferentiated mass of cells called a callus .
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In vitro and in response to specific cocktails of hormones (mainly auxins and cytokinins), most plant tissues can de-differentiate and form a mass of dividing totipotent stem cells called a callus. Organogenesis can then occur from those cells. The type of organ that is formed depends on the relative concentrations of the hormones in the medium.