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Artificial gene synthesis, or simply gene synthesis, refers to a group of methods that are used in synthetic biology to construct and assemble genes from nucleotides de novo. Unlike DNA synthesis in living cells, artificial gene synthesis does not require template DNA, allowing virtually any DNA sequence to be synthesized in the laboratory.
The 4R/2M assembly method was designed to combine parts (BioBrick Assembly Standard 10 or Silver Standard) within existing plasmids (i.e. without PCR or subcloning). The plasmids are reacted in vivo with sequence-specific DNA methyltransferases, so that each is modified and protected from one of two restriction endonucleases that are later used ...
In the field of cellular biology , single-cell analysis and subcellular analysis[ 1] is the study of genomics, transcriptomics, proteomics, metabolomics and cell–cell interactions at the single cell level. [ 2][ 3][ 4] The concept of single-cell analysis originated in the 1970s. Before the discovery of heterogeneity, single-cell analysis ...
This process of the second bacterial cell taking up new genetic material is called transformation. In molecular biology and genetics, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material from its surroundings through the cell membrane (s).
Assembly theory conceptualizes objects not as point particles, but as entities defined by their possible formation histories. [ 5] This allows objects to show evidence of selection, within well-defined boundaries of individuals or selected units. [ 5] Combinatorial objects are important in chemistry, biology and technology, in which most ...
Cytokinesis ( / ˌsaɪtoʊkɪˈniːsɪs /) is the part of the cell division process and part of mitosis during which the cytoplasm of a single eukaryotic cell divides into two daughter cells. Cytoplasmic division begins during or after the late stages of nuclear division in mitosis and meiosis.
BioArt. BioArt is an art practice where artists work with biology, live tissues, bacteria, living organisms, and life processes. Using scientific processes and practices such as biology and life science practices, microscopy, and biotechnology (including technologies such as genetic engineering, tissue culture, and cloning) the artworks are ...
Graph Assembly: is based on Graph theory in computer science. The de Bruijn Graph is an example of this approach and utilizes k-mers to assemble a contiguous from reads. [15] Greedy Graph Assembly: this approach score each added read to the assembly and selects the highest possible score from the overlapping region.