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The 4R/2M assembly method was designed to combine parts (BioBrick Assembly Standard 10 or Silver Standard) within existing plasmids (i.e. without PCR or subcloning). The plasmids are reacted in vivo with sequence-specific DNA methyltransferases, so that each is modified and protected from one of two restriction endonucleases that are later used ...
Artificial gene synthesis, or simply gene synthesis, refers to a group of methods that are used in synthetic biology to construct and assemble genes from nucleotides de novo. Unlike DNA synthesis in living cells, artificial gene synthesis does not require template DNA, allowing virtually any DNA sequence to be synthesized in the laboratory.
Biological illustration. Illustration from the book Histoire naturelle by Louis Renard, published in Amsterdam in 1754. Biological illustration is the use of technical illustration to visually communicate the structure and specific details of biological subjects of study. This can be used to demonstrate anatomy, explain biological functions or ...
In cell biology, the spindle apparatus is the cytoskeletal structure of eukaryotic cells that forms during cell division to separate sister chromatids between daughter cells. It is referred to as the mitotic spindle during mitosis, a process that produces genetically identical daughter cells, or the meiotic spindle during meiosis, a process ...
Assembly theory conceptualizes objects not as point particles, but as entities defined by their possible formation histories. [ 5] This allows objects to show evidence of selection, within well-defined boundaries of individuals or selected units. [ 5] Combinatorial objects are important in chemistry, biology and technology, in which most ...
A new study has found how a rose and other plants like a tomato and eggplant came to get their prickles. The discovery could help engineer new thorn-free variants.
Synthetic biology ( SynBio) is a multidisciplinary field of science that focuses on living systems and organisms, and it applies engineering principles to develop new biological parts, devices, and systems or to redesign existing systems found in nature. [ 1]
Graph Assembly: is based on Graph theory in computer science. The de Bruijn Graph is an example of this approach and utilizes k-mers to assemble a contiguous from reads. [15] Greedy Graph Assembly: this approach score each added read to the assembly and selects the highest possible score from the overlapping region.