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Artificial gene synthesis, or simply gene synthesis, refers to a group of methods that are used in synthetic biology to construct and assemble genes from nucleotides de novo. Unlike DNA synthesis in living cells, artificial gene synthesis does not require template DNA, allowing virtually any DNA sequence to be synthesized in the laboratory.
The 4R/2M assembly method was designed to combine parts (BioBrick Assembly Standard 10 or Silver Standard) within existing plasmids (i.e. without PCR or subcloning). The plasmids are reacted in vivo with sequence-specific DNA methyltransferases, so that each is modified and protected from one of two restriction endonucleases that are later used ...
Biological illustration. Illustration from the book Histoire naturelle by Louis Renard, published in Amsterdam in 1754. Biological illustration is the use of technical illustration to visually communicate the structure and specific details of biological subjects of study. This can be used to demonstrate anatomy, explain biological functions or ...
Gibson assembly is a molecular cloning method that allows for the joining of multiple DNA fragments in a single, isothermal reaction. It is named after its creator, Daniel G. Gibson, who is the chief technology officer and co-founder of the synthetic biology company, Telesis Bio. The technology is more efficient than manual plasmid genetic ...
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. [ 1] The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules.
Technical drawing, drafting or drawing, is the act and discipline of composing drawings that visually communicate how something functions or is constructed. Technical drawing is essential for communicating ideas in industry and engineering . To make the drawings easier to understand, people use familiar symbols, perspectives, units of ...
In the field of cellular biology , single-cell analysis and subcellular analysis[ 1] is the study of genomics, transcriptomics, proteomics, metabolomics and cell–cell interactions at the single cell level. [ 2][ 3][ 4] The concept of single-cell analysis originated in the 1970s. Before the discovery of heterogeneity, single-cell analysis ...
Genomics is an interdisciplinary field of molecular biology focusing on the structure, function, evolution, mapping, and editing of genomes.A genome is an organism's complete set of DNA, including all of its genes as well as its hierarchical, three-dimensional structural configuration.