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When starch is mixed with iodine in solution, an intensely dark blue colour develops, representing a starch/iodine complex. Starch is a substance common to most plant cells and so a weak iodine solution will stain starch present in the cells. Iodine is one component in the staining technique known as Gram staining, used in microbiology.
Gram stain (Gram staining or Gram's method), is a method of staining used to classify bacterial species into two large groups: gram-positive bacteria and gram-negative bacteria. It may also be used to diagnose a fungal infection. [1] The name comes from the Danish bacteriologist Hans Christian Gram, who developed the technique in 1884. [2]
Dispersion staining is an optical technique that results in a colored image of a colorless object. This is an optical staining technique and requires no stains or dyes to produce a color effect. There are five different microscope configurations used in the broader technique of dispersion staining.
In microscopy, negative staining is an established method, often used in diagnostic microscopy, for contrasting a thin specimen with an optically opaque fluid. In this technique, the background is stained, leaving the actual specimen untouched, and thus visible. This contrasts with positive staining, in which the actual specimen is stained.
Pages in category "Microbiology techniques" ... Time-lapse microscopy; Toluidine blue stain; Total viable count; Transmission electron microscopy DNA sequencing; V.
This technique, known as Gram staining, continues to be a standard procedure of medical microbiology. This work gained Gram an international reputation. The staining method later played a major role in classifying bacteria. Gram was a modest man, and in his initial publication he remarked, "I have therefore published the method, although I am ...
This technique can be used for the preparation of tissue of animals and plants. [14] For using under optical microscopy, the thickness of the material should be between above 2 and 25 micrometers. When observing under electron microscopy, sections should be from 20 to 30 nanometers. [2] Microtome can be used in sectioning of sufficiently thin ...
The Ziehl-Neelsen stain, also known as the acid-fast stain, is a bacteriological staining technique used in cytopathology and microbiology to identify acid-fast bacteria under microscopy, particularly members of the Mycobacterium genus.