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Human embryonic kidney 293 cells, also often referred to as HEK 293, HEK-293, 293 cells, are an immortalised cell line derived from HEK cells isolated from a female fetus in the 1970s. [ 1 ] [ 2 ] The HEK 293 cell line has been widely used in research for decades due to its reliable and fast growth and propensity for transfection .
In cellular biology, stable cells are cells that multiply only when needed. They spend most of the time in the quiescent G 0 phase of the cell cycle but can be stimulated to enter the cell cycle when needed. Examples include the liver, the proximal tubules of the kidney and endocrine glands.
There are several examples of immortalised cell lines, each with different properties. Most immortalised cell lines are classified by the cell type they originated from or are most similar to biologically 3T3 cells – a mouse fibroblast cell line derived from a spontaneous mutation in cultured mouse embryo tissue.
Cell engineering is the purposeful process of adding, deleting, or modifying genetic sequences in living cells to achieve biological engineering goals such as altering cell production, changing cell growth and proliferation requirements, adding or removing cell functions, and many more.
Early research (Bathurst and Jarrett, 1988) [13] found that cellular confinement reinforced gravel bases are "equivalent to about twice the thickness of unreinforced gravel bases" and that geocells performed better than single sheet reinforcement schemes (geotextiles and geogrids) and were more effective in reducing lateral spreading of infill under loading than conventional reinforced bases.
A cell bank is a facility that stores cells of specific genome for the purpose of future use in a product or medicinal needs, but can also describe the entity of stored cells itself. Cell banks often contain expansive amounts of base cell material that can be utilized for various projects.
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To save memory, the storage can be reduced to one array plus two line buffers. One line buffer is used to calculate the successor state for a line, then the second line buffer is used to calculate the successor state for the next line. The first buffer is then written to its line and freed to hold the successor state for the third line.