Search results
Results from the WOW.Com Content Network
The most commonly used fixative in histology is formaldehyde. It is usually used as a 10% neutral buffered formalin (NBF), that is approx. 3.7%–4.0% formaldehyde in phosphate buffer, pH 7. Since formaldehyde is a gas at room temperature, formalin – formaldehyde gas dissolved in water (~37% w/v) – is used when making the former fixative.
Formaldehyde (/ f ɔːr ˈ m æ l d ɪ h aɪ d / ⓘ for-MAL-di-hide, US also / f ə r-/ ⓘ fər-) (systematic name methanal) is an organic compound with the chemical formula CH 2 O and structure H−CHO, more precisely H 2 C=O.
The most widely used fixative for light microscopy is 10% neutral buffered formalin, or NBF (4% formaldehyde in phosphate buffered saline). [ 13 ] [ 12 ] [ 9 ] For electron microscopy, the most commonly used fixative is glutaraldehyde , usually as a 2.5% solution in phosphate buffered saline . [ 9 ]
Diff-Quik is a commercial Romanowsky stain variant used to rapidly stain and differentiate a variety of pathology specimens. It is most frequently used for blood films and cytopathological smears, including fine needle aspirates .
A mixture of these chemicals is known as embalming fluid and is used to preserve bodies of deceased persons for both funeral purposes and in medical research in anatomical laboratories. The period for which a body is embalmed is dependent on time, expertise of the embalmer and factors regarding duration of stay and purpose.
It is used most often in oncological surgery. [1] The technical name for this procedure is cryosection. The microtome device that cold cuts thin blocks of frozen tissue is called a cryotome. [2] The quality of the slides produced by frozen section is of lower quality than formalin fixed paraffin embedded tissue processing.
Here, the first priority is for long-term preservation, not presentation. As such, medical embalmers use anatomical wetting fluids that contain concentrated formaldehyde (37–40%, known as formalin) or glutaraldehyde and phenol, and are made without dyes or perfumes. Many embalming chemical companies make specialized anatomical embalming fluids.
Drug delivery systems have been around for many years, but there are a few recent applications of drug delivery that warrant 1. Drug delivery to the brain: Many drugs can be harmful when administered systemically; the brain is very sensitive to medications and can easily cause damage if a drug is administered directly into the bloodstream.