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Immunofluorescence image of HeLa cells grown in tissue culture and stained with antibody to actin in green, vimentin in red and DNA in blue Immunofluorescence of HeLa cells showing microtubules in green, mitochondria in yellow, nucleoli in red and nuclear DNA in purple. HeLa (/ ˈ h iː l ɑː /) is an immortalized cell line used in
English: HeLa cells stained with antibody to lysosomal LAMP1 in red and antibody to filament protein vimentin in green. Nuclear DNA shown in blue.
Immunofluorescence staining of HeLa Cells with antibody to reveal vimentin containing intermediate filaments in green and antibody to LAMP1 to reveal lysosomes in red. Nuclear DNA is seen in blue. Antibodies and image courtesy EnCor Biotechnology Inc. Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells.
Isolation from a naturally occurring cancer. This is the original method for generating an immortalised cell line. A major example is human HeLa, a line derived from cervical cancer cells taken on February 8, 1951 from Henrietta Lacks, a 31-year-old African-American mother of five, who died of cancer on October 4, 1951. [6]
These donor-specific HLA antibodies can exist pretransplant as consequence of sensitization to prior transplants or through pregnancies, but also occur de novo post-transplantation. There is a clear link between the risk of HLA antibody sensitisation and the donor-recipient HLA (molecular) mismatch.
A monoclonal antibody to HSP60 was used to stain human HeLa cells grown in tissue culture. The antibody reveals cellular mitochondria in red. The blue signal is due to a DNA binding dye which reveals cell nuclei. Antibody staining and image courtesy of EnCor Biotechnology Inc. Amino acid and structural sequence of HSP60 Protein. [12]
This is an accepted version of this page This is the latest accepted revision, reviewed on 24 January 2025. African-American woman (1920–1951), source of HeLa immortal cell line "Lacks" redirects here. For other uses, see Lack. Henrietta Lacks Lacks c. 1945–1951. Born Loretta Pleasant (1920-08-01) August 1, 1920 Roanoke, Virginia, U.S. Died October 4, 1951 (1951-10-04) (aged 31) Baltimore ...
RA33, also known as heterogeneous nuclear ribonucleoprotein A2/B1, is an autoantigen in human systemic autoimmune diseases.. In 1989, a novel class of autoantibodies was detected in sera of patients with rheumatoid arthritis (RA), which were directed against a protein with an estimated molecular mass of 33 kDa in nuclear extracts from HeLa cells. [1]
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