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There are many different ways to prepare PBS solutions, common ones are Dulbecco's phosphate-buffered saline (DPBS) [2] and the Cold Spring Harbor protocol. [3] Some formulations of DPBS do not contain potassium and magnesium, while other ones contain calcium and/or magnesium (depending on whether or not the buffer is used on live or fixed tissue: the latter does not require CaCl 2 or MgCl 2).
McIlvaine buffer is a buffer solution composed of citric acid and disodium hydrogen phosphate, also known as citrate-phosphate buffer. It was introduced in 1921 by the United States agronomist Theodore Clinton McIlvaine (1875–1959) from West Virginia University, and it can be prepared in pH 2.2 to 8 by mixing two stock solutions. [1]
Monopotassium phosphate (MKP) (also, potassium dihydrogen phosphate, KDP, or monobasic potassium phosphate) is the inorganic compound with the formula KH 2 PO 4. Together with dipotassium phosphate (K 2 HPO 4. (H 2 O) x) it is often used as a fertilizer, food additive, and buffering agent.
A buffer solution is a solution where the pH does not change significantly on dilution or if an acid or base is added at constant temperature. [1] Its pH changes very little when a small amount of strong acid or base is added to it. Buffer solutions are used as a means of keeping pH at a nearly constant value in a wide variety of chemical ...
The following table presents pK a values at 20 °C. Values change by about 0.01 per degree of temperature. [1] [3] Good's original 1966 paper had two older buffers (marked with italics) for comparison. In 1972 Good published a second list with three more buffers, and five more were added in 1980.
The tables below provides information on the variation of solubility of different substances ... 22.1: Potassium persulfate: K 2 S 2 O 8: 4.7: Potassium phosphate: K ...
Tripotassium phosphate, also called tribasic potassium phosphate [3] is a water-soluble salt with the chemical formula K 3 PO 4. (H 2 O) x (x = 0, 3, 7, 9). [ 4 ] Tripotassium phosphate is basic: a 1% aqueous solution has a pH of 11.8.
Potassium phosphate is a generic term for the salts of potassium and phosphate ions including: [1] Monopotassium phosphate (KH 2 PO 4) (Molar mass approx: 136 g/mol) Dipotassium phosphate (K 2 HPO 4) (Molar mass approx: 174 g/mol) Tripotassium phosphate (K 3 PO 4) (Molar mass approx: 212.27 g/mol) As food additives, potassium phosphates have ...
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