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The enzyme unit, or international unit for enzyme (symbol U, sometimes also IU) is a unit of enzyme's catalytic activity. [ 1 ] 1 U (μmol/min) is defined as the amount of the enzyme that catalyzes the conversion of one micro mole of substrate per minute under the specified conditions of the assay method .
The SI unit is the katal, 1 katal = 1 mol s −1 (mole per second), but this is an excessively large unit. A more practical and commonly used value is enzyme unit (U) = 1 μmol min −1 (micromole per minute). 1 U corresponds to 16.67 nanokatals. [1]
Only a narrow concentration of BSA is used (2-10 ug/mL) in order to create an accurate standard curve. [23] Using a broad range of protein concentration will make it harder to determine the concentration of the unknown protein. This standard curve is then used to determine the concentration of the unknown protein.
In essence, it is a sign that the transformation experiment was successful. [1] It should be determined under conditions of cell excess. [2] Transformation efficiency is typically measured as the number of transformed cells per total number of cells. It can be represented as a percentage or as colony forming units (CFUs) per microgram of DNA.
IC 50 is a quantitative measure that indicates how much of a particular inhibitory substance (e.g. drug) is needed to inhibit, in vitro, a given biological process or biological component by 50%. [1] The biological component could be an enzyme, cell, cell receptor or microbe. IC 50 values are typically expressed as molar concentration.
Therefore, it is common to equate 1 kilogram of water with 1 L of water. Consequently, 1 ppm corresponds to 1 mg/L and 1 ppb corresponds to 1 μg/L. Similarly, parts-per notation is used also in physics and engineering to express the value of various proportional phenomena. For instance, a special metal alloy might expand 1.2 micrometers per ...
Primer extension is a technique whereby the 5' ends of RNA can be mapped - that is, they can be sequenced and properly identified. Primer extension can be used to determine the start site of transcription (the end site cannot be determined by this method) by which its sequence is known.
The sample size is an important feature of any empirical study in which the goal is to make inferences about a population from a sample. In practice, the sample size used in a study is usually determined based on the cost, time, or convenience of collecting the data, and the need for it to offer sufficient statistical power. In complex studies ...