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Automated software package to annotate eukaryotic genes from RNA-Seq data and associated protein sequences Eukaryotes [1] FragGeneScan: Predicting genes in complete genomes and sequencing Reads: Prokaryotes, Metagenomes [2] ATGpr: Identifies translational initiation sites in cDNA sequences: Human [3] Prodigal
Salmon is a software tool for computing transcript abundance from RNA-seq data using either an alignment-free (based directly on the raw reads) or an alignment-based (based on pre-computed alignments) approach. It uses an online stochastic optimization approach to maximize the likelihood of the transcript abundances under the observed data.
The cost of high throughput DNA sequencing has reduced drastically from around $100 million/human genome in 2001 to around $1000/human genome in 2017. [3] Due to this increase in accessibility, high throughput DNA sequencing has become more widely used in research and clinical settings.
The sequences are then divided back into their original 11 bp tags using computer software in a process called deconvolution. [21] If a high-quality reference genome is available, these tags may be matched to their corresponding gene in the genome.
KEGG (Kyoto Encyclopedia of Genes and Genomes) is a collection of databases dealing with genomes, biological pathways, diseases, drugs, and chemical substances.KEGG is utilized for bioinformatics research and education, including data analysis in genomics, metagenomics, metabolomics and other omics studies, modeling and simulation in systems biology, and translational research in drug development.
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