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Colonial morphology serves as the first step in the identification of microbial species from clinical samples. [10] Based on the visual appearance of the colonies, microbiologists can narrow down the list of possible organisms, allowing them to select appropriate tests to provide a definitive diagnosis.
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Identification of non-annotated genes from sequences: MicrobesOnline relies on Glimmer [19] to automatically find genes in bacteria, archaea and viral sequences. Classification of proteins : The classification of proteins by their conserved domain, family and superfamilies determined by PIRSF, [ 20 ] Pfam , [ 21 ] SMART [ 22 ] and SUPERFAMILY ...
The aminopeptidase test analyzes bacteria for the production of the enzyme L-alanine-aminopeptidase, an enzyme found in many gram-negative bacteria. Adding L-Alanine-4-nitroanilide hydrochloride to a bacterial culture works as an indicator, changing to a yellow color in the presence of L-alanine-aminopeptidase.
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Ribotyping is a molecular technique for bacterial identification and characterization that uses information from rRNA-based phylogenetic analyses. [1] It is a rapid and specific method widely used in clinical diagnostics and analysis of microbial communities in food, water, and beverages.
Phage typing is a phenotypic method that uses bacteriophages ("phages" for short) for detecting and identifying single strains of bacteria. [1] Phages are viruses that infect bacteria and may lead to bacterial cell lysis. [2] The bacterial strain is assigned a type based on its lysis pattern. [3]
A molecular-weight size marker, also referred to as a protein ladder, DNA ladder, or RNA ladder, is a set of standards that are used to identify the approximate size of a molecule run on a gel during electrophoresis, using the principle that molecular weight is inversely proportional to migration rate through a gel matrix.