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PICRUSt [1] is a bioinformatics software package. The name is an abbreviation for Phylogenetic Investigation of Communities by Reconstruction of Unobserved States.. The tool serves in the field of metagenomic analysis where it allows inference of the functional profile of a microbial community based on marker gene survey along one or more samples.
Metatranscriptomics is the set of techniques used to study gene expression of microbes within natural environments, i.e., the metatranscriptome. [1]While metagenomics focuses on studying the genomic content and on identifying which microbes are present within a community, metatranscriptomics can be used to study the diversity of the active genes within such community, to quantify their ...
In 2020 Kang et al., using a strategy based on enrichment and dilution-to-extinction cultures, extracted from this original consortium (KMCG6) a simplified microbial consortia (SMC) with fewer species but similar keratinolytic activity. [23] Serial dilutions were performed on a keratinolytic microbial consortium pre-enriched from a soil sample.
T-RFLP Analysis (APLAUS+): Another ‚‘in-silico‘‘ assignment tool on the website of the Microbial Community Analysis project at the University of Idaho: BEsTRF: a tool for optimal resolution of terminal restriction fragment length polymorphism analysis based on user defined primer-enzyme-sequence databases
Metagenomic approaches to the analysis of complex microbial communities allow the targeted screening of enzymes with industrial applications in biofuel production, such as glycoside hydrolases. [84] Furthermore, knowledge of how these microbial communities function is required to control them, and metagenomics is a key tool in their understanding.
CAMERA, or the Community Cyberinfrastructure for Advanced Microbial Ecology Research and Analysis, is an online cloud computing service that provides hosted software tools and a high-performance computing infrastructure for the analysis of metagenomic data. [1] [2] [3] The project was announced in January 2006, becoming Calit2's flagship ...
Knowledge of the composition and metabolic activity of the microbiota in soils, water and waste materials is useful in optimizing crop production, in bioremediation and in understanding microbial ecosystems. Soil microbial community analysis by PLFA has been a widely used technique due to the sensitive, reproducible measurement of the dominant ...
Soil biology is the study of microbial and faunal activity and ecology in soil. Soil life, soil biota, soil fauna, or edaphon is a collective term that encompasses all organisms that spend a significant portion of their life cycle within a soil profile, or at the soil-litter interface.