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Electro-osmotic flow is most significant when in small channels, and is an essential component in chemical separation techniques, notably capillary electrophoresis. Electro-osmotic flow can occur in natural unfiltered water, as well as buffered solutions. Electro-osmotic flow schematic
Capillary electrophoresis is a separation technique which uses high electric field to produce electroosmotic flow for separation of ions. Analytes migrate from one end of capillary to other based on their charge, viscosity and size. Higher the electric field, greater is the mobility.
Capillary electrophoresis (CE) is a family of electrokinetic separation methods performed in submillimeter diameter capillaries and in micro- and nanofluidic channels.Very often, CE refers to capillary zone electrophoresis (CZE), but other electrophoretic techniques including capillary gel electrophoresis (CGE), capillary isoelectric focusing (CIEF), capillary isotachophoresis and micellar ...
[1] [2] Capillary electrochromatography is a combination of two analytical techniques, high-performance liquid chromatography and capillary electrophoresis. Capillary electrophoresis aims to separate analytes on the basis of their mass-to-charge ratio by passing a high voltage across ends of a capillary tube , which is filled with the analyte.
Electrophoresis is the basis for analytical techniques used in biochemistry and molecular biology to separate particles, molecules, or ions by size, charge, or binding affinity, either freely or through a supportive medium using a one-directional flow of electrical charge. [10]
If J v is positive, solvent is leaving the capillary (filtration). If negative, solvent is entering the capillary (absorption). Applying the classic Starling equation, it had long been taught that continuous capillaries filter out fluid in their arteriolar section and reabsorb most of it in their venular section, as shown by the diagram. [4]
Electroosmotic pumps are fabricated from silica nanospheres [6] [7] or hydrophilic porous glass, the pumping mechanism is generated by an external electric field applied on an electric double layer (EDL), generates high pressures (e.g., more than 340 atm (34 MPa) at 12 kV applied potentials) and high flow rates (e.g., 40 ml/min at 100 V in a pumping structure less than 1 cm 3 in volume).
When all of the TE ions are dissolved, the focusing process ceases and the analytes are separated according to the principles of zone electrophoresis. tITP is nowadays more widespread than conventional ITP because it is easily implemented in capillary electrophoresis (CE) separations as a preconcentration step, making CE more sensitive while ...