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In bioinformatics, BLAST (basic local alignment search tool) [3] is an algorithm and program for comparing primary biological sequence information, such as the amino-acid sequences of proteins or the nucleotides of DNA and/or RNA sequences.
A BLAST variant called MegaBLAST indexes 4 databases to speed up alignments. [ 9 ] BLAT can extend on multiple perfect and near-perfect matches (default is 2 perfect matches of length 11 for nucleotide searches and 3 perfect matches of length 4 for protein searches), while BLAST extends only when one or two matches occur close together.
formatdb is a discontinued software tool that was used in molecular bioinformatics to format protein or nucleotide databases for BLAST.It has been replaced by makeblastdb and the NCBI "strongly encourage[s]" [1] users to stop using formatdb.
In the FASTA method, the user defines a value k to use as the word length with which to search the database. The method is slower but more sensitive at lower values of k, which are also preferred for searches involving a very short query sequence. The BLAST family of search methods provides a number of algorithms optimized for particular types ...
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Next N is a command the will be able to go to the next indeterminate (N) nucleotide. Find in a File allows a user to search another file for selected sequences. Do BLAST Search command will perform a BLAST search in a separate web browser. The user may be able to either select certain date to BLAST or all sequences in the session will be used. [6]
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BLAST (biotechnology), Basic Local Alignment Search Tool, an algorithm used in bioinformatics; Blast cell or precursor cell, in cytology, a type of partially differentiated, usually unipotent cell; Blast disease, a disease of cereal crops; Blast injury, a complex type of physical trauma resulting from direct or indirect exposure to an explosion