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In biochemistry, denaturation is a process in which proteins or nucleic acids lose folded structure present in their native state due to various factors, including application of some external stress or compound, such as a strong acid or base, a concentrated inorganic salt, an organic solvent (e.g., alcohol or chloroform), agitation and radiation, or heat. [3]
Melting curve analysis is an assessment of the dissociation characteristics of double-stranded DNA during heating. As the temperature is raised, the double strand begins to dissociate leading to a rise in the absorbance intensity, hyperchromicity.
Denaturation Mapping is a form of optical mapping, first described in 1966. It is used to characterize DNA molecules without the need for amplification or sequencing . It is based on the differences between the melting temperatures of AT-rich and GC-rich regions. [ 1 ]
Nucleic acid melting curve showing hyperchromicity as a function of temperature. Hyperchromicity is the increase of absorbance (optical density) of a material.The most famous example is the hyperchromicity of DNA that occurs when the DNA duplex is denatured. [1]
Nucleic acid thermodynamics is the study of how temperature affects the nucleic acid structure of double-stranded DNA (dsDNA). The melting temperature (T m) is defined as the temperature at which half of the DNA strands are in the random coil or single-stranded (ssDNA) state.
Energy per unit temperature change J/K L 2 M T −2 Θ −1: extensive Heat flux density: ϕ Q: Heat flow per unit time per unit surface area W/m 2: M T −3: Illuminance: E v: Wavelength-weighted luminous flux per unit surface area lux (lx = cd⋅sr/m 2) L −2 J: Impedance: Z: Resistance to an alternating current of a given frequency ...
Denaturation (biochemistry), a structural change in macromolecules caused by extreme conditions; Denaturation (fissile materials), transforming fissile materials so that they cannot be used in nuclear weapons; Denaturation (food), intentional adulteration of food or drink rendering it unfit for consumption while remaining suitable for other uses
The process of determining a specific date (in years or some other unit of time) for an archaeological, geological or paleontological site or artifact. accident A sudden discontinuity of ground, such as a fault of great thickness, bed or lentil of unstable ground. [1] accretion A process by which material is added to a tectonic plate or ...