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In liquid chromatography, the mobile phase velocity is taken as the exit velocity, that is, the ratio of the flow rate in ml/second to the cross-sectional area of the ‘column-exit flow path.’ For a packed column, the cross-sectional area of the column exit flow path is usually taken as 0.6 times the cross-sectional area of the column.
Chromatographic peak resolution is given by = + where t R is the retention time and w b is the peak width at baseline. The bigger the time-difference and/or the smaller the bandwidths, the better the resolution of the compounds.
A high value for resolution corresponding to good separation of peaks is similar to the convention used with chromatography separations, [13] although it is important to note that the definitions are not the same. [14] High resolution indicating better peak separation is also used in ion mobility spectrometry. [15]
A mass chromatogram is a representation of mass spectrometry data as a chromatogram, where the x-axis represents time and the y-axis represents signal intensity. [1] The source data contains mass information; however, it is not graphically represented in a mass chromatogram in favor of visualizing signal intensity versus time.
(For instance, if the first column offers a peak capacity (k 1)of 100 for a 10-minute separation, and the second column offers a peak capacity of 5 (k 2) in a 5-second separation, then the combined peak capacity may approach k 1 × k 2 =500, with the total separation time still ~ 10 minutes). 2D separations have been applied to the analysis of ...
President-elect Donald Trump and his team are working behind closed doors at Mar-a-Lago to staff the 47th president's administration, with loyalty the primary job requirement.
This past summer, Sacramento had its hottest 20-day stretch on record, averaging 103.8 degrees during those three weeks from June 23 to July 12. On July 5, the temperature reached 110. The next ...
“Wicked” costume designer Paul Tazewell opens up about the making of Elphaba and Glinda's costumes on Wicked — the meaning of the Elphaba's back outfits, the bubble dress and more.