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A transcription factor is a protein that binds to specific DNA sequences (enhancer or promoter), either alone or with other proteins in a complex, to control the rate of transcription of genetic information from DNA to messenger RNA by promoting (serving as an activator) or blocking (serving as a repressor) the recruitment of RNA polymerase.
The protein version of the transcription factor is detectable by using specific antibodies. The sample is detected on a western blot. By using electrophoretic mobility shift assay (EMSA), [88] the activation profile of transcription factors can be detected.
Transcription factor TF II A is a nuclear protein involved in the RNA polymerase II-dependent transcription of DNA. [1] TF II A is one of several general (basal) transcription factors ( GTFs ) that are required for all transcription events that use RNA polymerase II.
Download as PDF; Printable version; In other projects ... Diageram of transcription factors. Transcription factor SPT20 is a ... SAGA is required to engage basal ...
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals.
Control of transcription by RNA polymerase II involves the basal transcription machinery, which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes that are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site.
TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation.
[2] [4] [5] It has been shown that DPE-dependent basal transcription depends highly on the Inr (and vice versa) and on correct spacing between the two elements. [ 2 ] [ 4 ] [ 6 ] The DPE consensus sequence was originally thought to be RGWCGTG, [ 2 ] however more recent studies have suggested it to be the similar but more general sequence RGWYV(T).