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[4]: 114 A DataFrame is a 2-dimensional data structure of rows and columns, similar to a spreadsheet, and analogous to a Python dictionary mapping column names (keys) to Series (values), with each Series sharing an index. [4]: 115 DataFrames can be concatenated together or "merged" on columns or indices in a manner similar to joins in SQL.
Column chromatography in chemistry is a chromatography method used to isolate a single chemical compound from a mixture. Chromatography is able to separate substances based on differential absorption of compounds to the adsorbent; compounds move through the column at different rates, allowing them to be separated into fractions.
Finally, elution is the process of adding an aqueous solution to the column, allowing the hydrophilic nucleic acid to leave the column and return to solution. This step may be improved with salt, pH, time, or heat. Finally, to capture the eluate/eluent, the column is transferred into a clean microtube prior to a last centrifugation step.