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Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues, or organs under sterile conditions on a nutrient culture medium of known composition. It is widely used to produce clones of a plant in a method known as micropropagation .
Somatic embryogenesis is an artificial process in which a plant or embryo is derived from a single somatic cell. [1] Somatic embryos are formed from plant cells that are not normally involved in the development of embryos, i.e. ordinary plant tissue. No endosperm or seed coat is formed around a somatic embryo.
The plant tissues are removed from an intact plant in a sterile condition. Clean stock materials that are free of viruses and fungi are important in the production of the healthiest plants. Once the plant material is chosen for culture, the collection of explant(s) begins and is dependent on the type of tissue to be used, including stem tips ...
Structure of a plant cell. Plant cells are the cells present in green plants, photosynthetic eukaryotes of the kingdom Plantae.Their distinctive features include primary cell walls containing cellulose, hemicelluloses and pectin, the presence of plastids with the capability to perform photosynthesis and store starch, a large vacuole that regulates turgor pressure, the absence of flagella or ...
These cells may be cells isolated from a donor organism (primary cells) or an immortalised cell line. The cells are bathed in a culture medium, which contains essential nutrients and energy sources necessary for the cells' survival. [8] Thus, in its broader sense, "tissue culture" is often used interchangeably with "cell culture".
The structure of a plant cell wall is incredibly important for wound responses, as both protect the plant from pathogenic infections by preventing various molecules from entering the cell. [ 1 ] Plants are capable of activating innate immunity, by responding to wounding events with damage-associated Molecular Patterns (DAMPs). [ 1 ]
The meristem can be defined as the tissue or group of plant tissues that contain undifferentiated stem cells, which are capable of producing any type of cell tissue.Their maintenance and development, both in the vegetative meristem or the meristem of the inflorescence is controlled by genetic cell fate determination mechanisms.
4) The new cell wall of the daughter cells connects with the parent cell wall along the line of the former preprophase band location. The preprophase band is a microtubule array found in plant cells that are about to undergo cell division and enter the preprophase stage of the plant cell cycle .