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Blood agar plates (BAPs) contain mammalian blood (usually sheep or horse), typically at a 5–10% concentration. BAPs are enriched, and differential media is used to isolate fastidious organisms and detect hemolytic activity. β-Hemolytic activity will show lysis and complete digestion of red blood cell contents surrounding a colony.
Mannitol salt agar or MSA is a commonly used selective and differential growth medium in microbiology. It encourages the growth of a group of certain bacteria while inhibiting the growth of others. [ 1 ]
E. coli on EMB agar. Eosin methylene blue (EMB, also known as "Levine's formulation") is a selective and differential media used for the identification of Gram-negative bacteria, [1] specifically the Enterobacteriaceae. EMB inhibits the growth of most Gram-positive bacteria. EMB is often used to confirm the presence of coliforms in a sample.
Bile salts are the selective ingredient, while esculin is the differential component. Enterococcus hydrolyze esculin to products that react with ferric citrate in the medium to produce insoluble iron salts, resulting in the blackening of the medium. Test results must be interpreted in conjunction with gram stain morphology.
MacConkey agar is a selective and differential culture medium for bacteria. It is designed to selectively isolate gram-negative and enteric (normally found in the intestinal tract) bacteria and differentiate them based on lactose fermentation. [1] Lactose fermenters turn red or pink on MacConkey agar, and nonfermenters do not change color.
Hoyle's agar is a selective medium that uses tellurite to differentially select Corynebacterium diphtheriae from other upper respiratory tract flora. The medium appears cream to yellow colored, and takes the form of a free-floating powder. It is a modification of Neill's medium. [1] Hoyle's tellurite agar contains:
De Man–Rogosa–Sharpe agar, often abbreviated to MRS, is a selective culture medium designed to favour the luxuriant growth of Lactobacilli for lab study. Developed in 1960, this medium was named for its inventors, Johannes Cornelis de Man [ Wikidata ] , Morrison Rogosa [ Wikidata ] , and Margaret Elisabeth Sharpe [ Wikidata ] .
To transfer cells from a sample to the agar, a sterilized needle is used to select a distinct colony from the sample and to streak across the agar surface, as is done on an agar plate. It is important to prepare a light cell inoculum because carry-over of nutrients [ 9 ] or dead bacterial cell matter containing carbon and nitrogen from the ...