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Aerococcus urinae is a member of the bacterial genus Aerococcus.The bacterium is a Gram-positive, catalase-negative coccus growing in clusters. Isolates of this genus were originally isolated in 1953 from samples collected in the air and dust of occupied rooms and were distinguished by their tetrad cellular arrangements. [2]
Gram stain (Gram staining or Gram's method), is a method of staining used to classify bacterial species into two large groups: gram-positive bacteria and gram-negative bacteria. It may also be used to diagnose a fungal infection. [1] The name comes from the Danish bacteriologist Hans Christian Gram, who developed the technique in 1884. [2]
Violet-stained gram-positive cocci and pink-stained gram-negative bacilli. In bacteriology, gram-positive bacteria are bacteria that give a positive result in the Gram stain test, which is traditionally used to quickly classify bacteria into two broad categories according to their type of cell wall.
These bacteria are Gram-positive and strictly anaerobic. [2] [3] [4] The genus Anaerococcus was proposed in 2001. [2] [4] Its genome was sequenced in August 2009. The genus Anaerococcus is one of six genera classified within the group GPAC (Gram-Positive Anaerobic Cocci). [5]
Members of the genus Macrococcus are Gram-positive, nonmotile, non-spore-forming cocci that are coagulase negative and catalase positive. They can be distinguished phenotypically from most staphylococci on the basis of their cellular morphology (they are 2.5 – 4.0 times larger in diameter compared to Staphylococcus aureus) and their positive cytochrome c oxidase reaction.
Streptococcus mitis is a species of Gram-positive, mesophilic, alpha-hemolytic bacteria in the genus Streptococcus.These bacteria are facultative anaerobes, and made up of non-motile and non-sporing cocci (round cells) that are catalase negative.
Perform Gram-stain to identify the bacterium in question, continue to step 2 if it is found to be Gram negative cocci. Neisseria flava is an aerobic microbe, try growing some of the same in the presence in air/air + CO 2. Transfer sample able to growth in air to a NA medium at 25C, continue to step 4 if colonies are observed.
A Gram stain of a urethral exudate showing typical intracellular Gram-negative diplococci, which is diagnostic for gonococcal urethritis [17]. Neisseria species are fastidious, Gram-negative cocci (though some species are rod-shaped and occur in pairs or short chains) that require nutrient supplementation to grow in laboratory cultures. [18]