Search results
Results from the WOW.Com Content Network
A semi-automated method for measuring the average length of telomeres with Flow FISH was published in Nature Protocols in 2006. [56] While multiple companies offer telomere length measurement services, the utility of these measurements for widespread clinical or personal use has been questioned.
The telomere was first discovered by biologist Hermann Joseph Muller in the early 20th century. [4] However, experiments by Elizabeth Blackburn, Carol Greider, and Jack Szostak in the 1980s led to the successful discovery of telomerase (the enzyme responsible for maintaining telomere length) and a better understanding of telomeres. [5] [6] [7]
Flow-FISH was first published in 1998 by Rufer et al. [11] as a modification of another technique for analyzing telomere length, Q-FISH, that employs peptide nucleic acid probes [12] of a 3'-CCCTAACCCTAACCCTAA-5' sequence labeled with a fluorescin fluorophore to stain telomeric repeats on prepared metaphase spreads of cells that have been treated with colcemid, hypotonic shock, and fixation to ...
Measurements of telomere lengths across cell types at various ages suggest that this gradual chromosome shortening results in a gradual reduction in telomere length at a rate of approximately 25 nucleotides per year. [3]
In flow-FISH, flow cytometry is utilized to measure fluorescence intensity (and thus telomere length) in a large population of cells rather than just a handful of cells in Q-FISH. Conversely, unlike Q-FISH, flow-FISH is unable to determine telomere length in a particular chromosome within an individual cell. [ 5 ]
Life Length [2] is a biotechnology company. [3] Located in Madrid, it provides telomere diagnostics as well as telomerase measurement. [4] [5]It was founded by American entrepreneur Stephen J. Matlin and Dr. María Blasco Marhuenda [6] in 2010 with the objective to commercialize Blasco's HT Q-FISH conceptual work.
In a world of fitness trackers built to optimize, the greatest indicators of longevity aren't what you expect.
He subsequently showed that flow cytometry can be used to measure the average telomere length in nucleated blood cells. [14] This so-called flow FISH technique is now the method of choice to screen for possible telomere biology disorders in humans and such tests are offered by Repeat Diagnostics Inc., a biotech company founded by Lansdorp.