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Chemically modified versions of biotin are widely used throughout the biotechnology industry to isolate proteins and non-protein compounds for biochemical assays. [33] Because egg-derived avidin binds strongly to biotin with a dissociation constant K d ≈ 10 −15 M, [ 34 ] biotinylated compounds of interest can be isolated from a sample by ...
Some top dietary sources of biotin include: Beef Liver: While you should consume red meat in moderation, beef liver offers 103% of the Daily Value for biotin with 31 micrograms per 3-ounce serving.
Also, multiple biotin molecules can be conjugated to a protein of interest, which allows binding of multiple streptavidin, avidin or neutravidin protein molecules and increases the sensitivity of detection of the protein of interest. There is a large number of biotinylation reagents available that exploit the wide range of possible labelling ...
Biotin - Avidin can bind up to four molecules of biotin simultaneously with a high degree of affinity and specificity. Avidin is a tetrameric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. Dimeric members of the avidin family are also found in some bacteria. [1]
Biotin is an essential B vitamin that helps the body convert food into energy and supports skin and hair health. Add these 7 biotin-rich foods to your diet for benefits.
This high-protein food doubles as a biotin-rich food. One 3-ounce serving of canned tuna (in water) offers up 2% the daily recommended biotin, according to the National Institutes of Health. It ...
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