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Helicase is an essential component of cellular mechanisms that ensures accurate DNA replication and maintenance of genetic information. DNA helicase catalyzes regression. RecG and the enzyme PriA work together to rewind duplex DNA, creating a Holliday junction.
RecBCD is unusual amongst helicases because it has two helicases that travel with different rates [6] and because it can recognize and be altered by the Chi DNA sequence. [7] [8] RecBCD avidly binds an end of linear double-stranded (ds) DNA. The RecD helicase travels on the strand with a 5' end at which the enzyme initiates unwinding, and RecB ...
RecQ helicase is a family of helicase enzymes initially found in Escherichia coli [1] that has been shown to be important in genome maintenance. [ 2 ] [ 3 ] [ 4 ] They function through catalyzing the reaction ATP + H 2 O → ADP + P and thus driving the unwinding of paired DNA and translocating in the 3' to 5' direction.
The two pathways for homologous recombination in eukaryotes, showing the formation and resolution of Holliday junctions. The Holliday junction is a key intermediate in homologous recombination, a biological process that increases genetic diversity by shifting genes between two chromosomes, as well as site-specific recombination events involving integrases.
This model is based on reactions of DNA and RecBCD with ATP in excess over Mg2+ ions. Step 1: RecBCD binds to a double-stranded DNA end. Step 2: RecBCD unwinds DNA. RecD is a fast helicase on the 5’-ended strand, and RecB is a slower helicase on the 3'-ended strand (that with an arrowhead) [ref 46 in current Wiki version].
When the progress of the replication fork is impeded the proteins single-stranded binding protein SSB and RecG helicase along with the RuvABC complex are required for rescue. [2] The resolution of Holliday junctions that accumulate following replication on damaged DNA templates in E. coli requires the RuvABC complex. [3]
Helicases in eukaryotic cells are remarkably complex. [106] The catalytic core of the helicase is composed of six minichromosome maintenance (Mcm2-7) proteins, forming a hexameric ring. Away from DNA, the Mcm2-7 proteins form a single heterohexamer and are loaded in an inactive form at origins of DNA replication as a head-to-head double ...
The RecF pathway, also called the RecFOR pathway, is a pathway of homologous recombination that repairs DNA in bacteria.It repairs breaks that occur on only one of DNA's two strands, known as single-strand gaps.