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Gel permeation chromatography (GPC) [1] is a type of size-exclusion chromatography (SEC), that separates high molecular weight or colloidal analytes on the basis of size or diameter, typically in organic solvents. The technique is often used for the analysis of polymers.
Some of the most common methods for determining these parameters are colligative property measurements, static light scattering techniques, viscometry, and size exclusion chromatography. Gel permeation chromatography, a type of size exclusion chromatography, is an especially useful technique used to directly determine the molecular weight ...
Size-exclusion chromatography, also known as molecular sieve chromatography, [1] is a chromatographic method in which molecules in solution are separated by their shape, and in some cases size. [2] It is usually applied to large molecules or macromolecular complexes such as proteins and industrial polymers . [ 3 ]
Size-exclusion chromatography (SEC) is also known as gel permeation chromatography (GPC) or gel filtration chromatography and separates molecules according to their size (or more accurately according to their hydrodynamic diameter or hydrodynamic volume). Smaller molecules are able to enter the pores of the media and, therefore, molecules are ...
Gel permeation chromatography (also known as size-exclusion chromatography) Light scattering measurements such as dynamic light scattering; Direct measurement via mass spectrometry, using matrix-assisted laser desorption/ionization (MALDI) or electrospray ionization with tandem mass spectrometry (ESI-MS/MS)
Size-exclusion chromatography (SEC) [30] separates polymer molecules and biomolecules based on differences in their molecular size (actually by a particle's Stokes radius). The separation process is based on the ability of sample molecules to permeate through the pores of gel spheres, packed inside the column, and is dependent on the relative ...
In analytical chemistry applied to polymers and sometimes small molecules tortuosity is applied in gel permeation chromatography (GPC) also known as size exclusion chromatography (SEC). As with any chromatography it is used to separate mixtures.
These flow mode MALS measurements have been extended to other separation techniques such as field flow fractionation, ion exchange chromatography, and reversed-phase chromatography. The angular dependence of light scattering data is shown below in a figure of mix of polystyrene spheres which was separated by SEC.