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In microbiology, the term isolation refers to the separation of a strain from a natural, mixed population of living microbes, as present in the environment, for example in water or soil, or from living beings with skin flora, oral flora or gut flora, in order to identify the microbe(s) of interest. [1]
Bacteria (/ b æ k ˈ t ɪər i ə / ⓘ; sg.: bacterium) are ubiquitous, mostly free-living organisms often consisting of one biological cell.They constitute a large domain of prokaryotic microorganisms.
Corynebacterium (/ k ɔː ˈ r aɪ n ə b æ k ˌ t ɪər i ə m,-ˈ r ɪ n-/) is a genus of Gram-positive bacteria and most are aerobic.They are bacilli (rod-shaped), and in some phases of life they are, more specifically, club-shaped, which inspired the genus name (coryneform means "club-shaped").
Aerococcus urinae is a member of the bacterial genus Aerococcus.The bacterium is a Gram-positive, catalase-negative coccus growing in clusters. Isolates of this genus were originally isolated in 1953 from samples collected in the air and dust of occupied rooms and were distinguished by their tetrad cellular arrangements. [2]
Aspergillus sp. growing in potato dextrose agar Potato dextrose agar (BAM Media M127) and potato dextrose broth are common microbiological growth media made from potato infusion and dextrose. Potato dextrose agar (abbreviated "PDA") is the most widely used medium for growing fungi and bacteria. PDA has the capability to culture various bacteria and fungi found in the soil. This agar can be ...
In 1974, a novel bacterium was isolated from the skin of a healthy adult woman. The novel species was originally named Micrococcus kristinae, and was named after the person from which it was first isolated (Kristin Holding). [2]
Typical genera include: [3] Citrobacter are peritrichous facultative anaerobic bacilli between 0.6–6 μm in length. [4] Citrobacter species inhabit intestinal flora without causing harm, but can lead to urinary tract infections, bacteremia, brain abscesses, pneumonia, intra abdominal sepsis, meningitis, and joint infections if they are given the opportunity. [4]
Microbial cultures on solid and liquid media. A microbiological culture, or microbial culture, is a method of multiplying microbial organisms by letting them reproduce in predetermined culture medium under controlled laboratory conditions.