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Tissue image cytometry or tissue cytometry [1] is a method of digital histopathology and combines classical digital pathology (glass slides scanning and virtual slide generation) and computational pathology (digital analysis) into one integrated approach with solutions for all kinds of diseases, tissue and cell types as well as molecular markers and corresponding staining methods to visualize ...
Since patient samples are assembled into the same block, sections can be stained with the same protocol to avoid experimental variability and technical artefacts. Clinical cancer patient cohorts and corresponding tissue microarray sets have been used to study diagnostic, prognostic and treatment predictive cancer biomarkers in most forms of ...
This is a staining method to illustrates mineralization such as calcium and potassium in tissues. [1] [2] [3] [4] [5]It is a precipitation reaction in which silver ...
The most commonly used stain in histology is a combination of hematoxylin and eosin (often abbreviated H&E). Hematoxylin is used to stain nuclei blue, while eosin stains the cytoplasm and the extracellular connective tissue matrix of most cells pink. There are hundreds of various other techniques which have been used to selectively stain cells.
Another common variant is the Masson trichrome & Verhoeff stain, which combines the Masson trichrome stain and Verhoeff's stain. [2] This combination is useful for the examination of blood vessels ; the Verhoeff stain highlights elastin (black) and allows one to easily differentiate small arteries (which typically have at least two elastic ...
In histology, histopathology, and clinical pathology, Perls Prussian blue is a commonly used method to detect the presence of iron in tissue or cell samples. [1]: 235 [2] [3] [4] Perls Prussian Blue derives its name from the German pathologist Max Perls (1843–1881), who described the technique in 1867. [2]
Trichrome staining is a histological staining method that uses two or more acid dyes in conjunction with a polyacid. Staining differentiates tissues by tinting them in contrasting colours. It increases the contrast of microscopic features in cells and tissues, which makes them easier to see when viewed through a microscope.
The H&E staining procedure is the principal stain in histology [3] [7] [2] [5] in part because it can be done quickly, [7] is not expensive, and stains tissues in such a way that a considerable amount of microscopic anatomy [9] [10] is revealed, [7] [5] [4] and can be used to diagnose a wide range of histopathologic conditions. [8]