Ad
related to: lipid staining dyes examples in medicine namesbroadpharm.com has been visited by 10K+ users in the past month
Search results
Results from the WOW.Com Content Network
A lysochrome is a soluble dye used for histochemical staining of lipids, which include triglycerides, fatty acids, and lipoproteins. Lysochromes such as Sudan IV dissolve in the lipid and show up as colored regions. The dye does not stick to any other substrates, so a quantification or qualification of lipid presence can be obtained.
Oil Red O (Solvent Red 27, Sudan Red 5B, C.I. 26125, C 26 H 24 N 4 O) is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. It has the appearance of a red powder with an absorbance maximum at 518 nanometers. [1]
Sudan stain test is often used to determine the level of fecal fat to diagnose steatorrhea. A small sample is dissolved in water or saline, glacial acetic acid is added to hydrolyze the insoluble salts of fatty acids , a few drops of alcoholic solution of Sudan III are added, the sample is spread on a microscopic slide, and heated twice to boil.
Sudan III is a dye used for Sudan staining. Similar dyes include Oil Red O, Sudan IV, and Sudan Black B. They are used for staining of triglycerides in frozen sections, and some protein bound lipids and lipoproteins on paraffin sections. It has the appearance of reddish brown crystals and a maximum absorption at 507(304) nm. [6]
A Ziehl–Neelsen stain is an acid-fast stain used to stain species of Mycobacterium tuberculosis that do not stain with the standard laboratory staining procedures such as Gram staining. This stain is performed through the use of both red coloured carbol fuchsin that stains the bacteria and a counter stain such as methylene blue .
Sudan IV (C 24 H 20 N 4 O) is a lysochrome (fat-soluble dye) diazo dye used for the staining of lipids, triglycerides and lipoproteins on frozen paraffin sections. It has the appearance of reddish brown crystals with melting point 199 °C and maximum absorption at 520(357) nm.
Common name Synonyms C.I. generic name C.I. number Class CAS number Acid Black 52 Acid Black 52 15711 azo 5610-64-0: Acid Blue AS Weak Acid Blue Acid Blue 25: 62055 anthraquinone: 6408-78-2: Acid fuchsin: Acid Magenta Acid Rubin Acid Violet 19 42685 triarylmethane: 3244-88-0: Acid orange 3 Acid orange 3 10385 nitro 6373-74-6: Acid orange 19 ...
[2] [3] Carbol fuchsin is used as the primary stain dye to detect acid-fast bacteria because it is more soluble in the cells' wall lipids than in the acid alcohol. If the bacteria is acid-fast the bacteria will retain the initial red color of the dye because they are able to resist the destaining by acid alcohol (0.4–1% HCl in 70% EtOH). [4]
Ad
related to: lipid staining dyes examples in medicine namesbroadpharm.com has been visited by 10K+ users in the past month