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URA3 is often used in yeast research as a "marker gene", that is, a gene to label chromosomes or plasmids. URA3 encodes Orotidine 5'-phosphate decarboxylase (ODCase) , which is an enzyme that catalyzes one reaction in the synthesis of pyrimidine ribonucleotides (a component of RNA ).
In yeast and bacteria, OMP decarboxylase is a single-function enzyme.However, in mammals, OMP decarboxylase is part of a single protein with two catalytic activities.This bifunctional enzyme is named UMP synthase and it also catalyzes the preceding reaction in pyrimidine nucleotide biosynthesis, the transfer of ribose 5-phosphate from 5-phosphoribosyl-1-pyrophosphate to orotate to form OMP.
5-Fluoroorotic acid (5FOA) is a fluorinated derivative of the pyrimidine precursor orotic acid.It is used in yeast genetics to select for the absence of the URA3 gene, which encodes the enzyme for the decarboxylation of 5-fluoroorotic acid to 5-fluorouracil, a toxic metabolite. [1]
The yeast deletion project, formally the Saccharomyces Genome Deletion Project, is a project to create data for a near-complete collection of gene-deletion mutants of the yeast Saccharomyces cerevisiae. Each strain carries a precise deletion of one of the genes in the genome. This allows researchers to determine what each gene does by comparing ...
Y187 is a strain of yeast (Saccharomyces cerevisiae) used in biological research for two-hybrid screening.The strain has been sold commercially by Clontech [1] since at least 2000 and is used as a partner with strain AH109 in mating assays.
Seed oils — plant-based cooking oils often used in processed, packaged foods — have been linked to an increased risk of colon cancer, according to a new study published in the medical journal Gut.
Gene knockdown is an experimental technique by which the expression of one or more of an organism's genes is reduced. The reduction can occur either through genetic modification or by treatment with a reagent such as a short DNA or RNA oligonucleotide that has a sequence complementary to either gene or an mRNA transcript.
In a BMJ study from 2014, for instance, researchers evaluated health claims made on 40 randomly selected episodes of “The Dr. Oz Show,” a syndicated daytime TV show that ran from 2009 to 2022 ...