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Giemsa's solution is a mixture of methylene blue, eosin, and Azure B.The stain is usually prepared from commercially available Giemsa powder. A thin film of the specimen on a microscope slide is fixed in pure methanol for 30 seconds, by immersing it or by putting a few drops of methanol on the slide.
The Gimenez staining technique uses biological stains to detect and identify bacterial infections in tissue samples. Although largely superseded by techniques like Giemsa staining , the Gimenez technique may be valuable for detecting certain slow-growing or fastidious bacteria.
The staining technique using Giemsa staining is called G banding and therefore produces the typical "G-Bands". [ 12 ] A full account of a karyotype may therefore include the number, type, shape and banding of the chromosomes, as well as other cytogenetic information.
The fixative is essential for good staining and presentation of cellular detail. After fixation, the slide is stained to distinguish the cells from each other. [citation needed] Routine analysis of blood in medical laboratories is usually performed on blood films stained with Romanowsky stains such as Wright's stain, Giemsa stain, or Diff-Quik ...
G-banding, G banding or Giemsa banding is a technique used in cytogenetics to produce a visible karyotype by staining condensed chromosomes. It is the most common chromosome banding method. [ 1 ] It is useful for identifying genetic diseases (mainly chromosomal abnormalities ) through the photographic representation of the entire chromosome ...
[1] [2] [3] The Diff-Quik procedure is based on a modification of the Wright-Giemsa stain pioneered by Harleco in the 1970s, [1] and has advantages over the routine Wright-Giemsa staining technique in that it reduces the 4-minute process into a much shorter operation and allows for selective increased eosinophilic or basophilic staining ...
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Stain w/ Giemsa, methylene blue or Wright’s stain. Microscopic examination using an oil immersion lens. (Look for multinucleated giant cells) [7] A modified test can be performed using proprietary agents which requires fewer steps and allows the sample to be fixed quicker.