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The method is used to identify a single-nucleotide polymorphism (SNP). In the method, an oligonucleotide primer hybridizes to a complementary region along the nucleic acid to form a duplex, with the primer’s terminal 3’-end directly adjacent to the nucleotide base to be identified. Using a DNA polymerase, the oligonucleotide primer is ...
Primer extension can be used to determine the start site of transcription (the end site cannot be determined by this method) by which its sequence is known. This technique requires a radiolabelled primer (usually 20 - 50 nucleotides in length) which is complementary to a region near the 3' end of the mRNA.
The basic procedure requires the synthesis of a short DNA primer. This synthetic primer contains the desired mutation and is complementary to the template DNA around the mutation site so it can hybridize with the DNA in the gene of interest. The mutation may be a single base change (a point mutation), multiple base changes, deletion, or insertion.
Smokeless powder may be corned into small spherical balls or extruded into cylinders or strips with many cross-sectional shapes (strips with various rectangular proportions, single or multi-hole cylinders, slotted cylinders) using solvents such as ether. These extrusions can be cut into short ("flakes") or long pieces ("cords" many inches long).
Detonating cord (also called detonation cord, detcord, detacord, blasting rope, or primer cord) is a thin, flexible plastic tube usually filled with pentaerythritol tetranitrate (PETN, pentrite). With the PETN exploding at a rate of approximately 6,400 m/s (21,000 ft/s), any common length of detonation cord appears to explode instantaneously.
The last 10-12 bases at the 3' end of a primer are sensitive to initiation of polymerase extension and general primer stability on the template binding site. The effect of a single mismatch at these last 10 bases at the 3' end of the primer depends on its position and local structure, reducing the primer binding, selectivity, and PCR efficiency.
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