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In centrifugation the clearing factor or k factor represents the relative pelleting efficiency of a given centrifuge rotor at maximum rotation speed. It can be used to estimate the time t {\displaystyle t} (in hours) required for sedimentation of a fraction with a known sedimentation coefficient s {\displaystyle s} (in svedbergs ):
A standard ultracentrifuge by manufacturer Beckman Coulter. An ultracentrifuge is a centrifuge optimized for spinning a rotor at very high speeds, capable of generating acceleration as high as 1 000 000 g (approx. 9 800 km/s²). [1] There are two kinds of ultracentrifuges, the preparative and the analytical ultracentrifuge.
Beckman Coulter, Inc. is a Danaher Corporation company that develops, manufactures, and markets products relevant to biomedical testing. It operates in the industries of diagnostics and life sciences .
A centrifuge can be a very effective filter that separates contaminants from the main body of fluid. Industrial scale centrifuges are commonly used in manufacturing and waste processing to sediment suspended solids, or to separate immiscible liquids. An example is the cream separator found in dairies.
A tricanter centrifuge operates on a similar principle to decanter centrifuges but instead separates three phases, consisting of a suspended solids phase and two immiscible liquids. [5] Sedimentation of the suspended solids occurs as normal where they accumulate on the wall of the bowl and are conveyed out of the centrifuge.
Centrifugation is a mechanical process which involves the use of the centrifugal force to separate particles from a solution according to their size, shape, density, medium viscosity and rotor speed. [1]
Arnold Orville Beckman (April 10, 1900 – May 18, 2004) was an American chemist, inventor, investor, and philanthropist. While a professor at California Institute of Technology, he founded Beckman Instruments based on his 1934 invention of the pH meter, a device for measuring acidity (and alkalinity), later considered to have "revolutionized the study of chemistry and biology". [1]
Thus, the differential centrifugation method is the successive pelleting of particles from the previous supernatant, using increasingly higher centrifugation forces. [3] Cellular organelles separated by differential centrifugation maintain a relatively high degree of normal functioning, as long as they are not subject to denaturing conditions ...
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