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Isolation from a naturally occurring cancer. This is the original method for generating an immortalised cell line. A major example is human HeLa, a line derived from cervical cancer cells taken on February 8, 1951 from Henrietta Lacks, a 31-year-old African-American mother of five, who died of cancer on October 4, 1951. [6]
However, the cell line is appreciated to be N-type (neuronal), given its morphology and the ability to differentiate the cells into along the neuronal lineage (in contrast to the S-type SH-EP subcloned cell line, also derived from SK-N-SH). [4] Cells with short spiny neurite-like processes migrate out from these adherent clumps. SH-SY5Y cells ...
Supposed cell line Existent? Supposed cell type Real cell line Real cell type Reference Cellosaurus; 207: Existent: pre-B cell leukemia: REH or CCRF-CEM: pre-B cell or T cell leukemia [7] CVCL_K034: 2474/90: Virtual: stomach carcinoma: HT-29: colon carcinoma [7] CVCL_9556: 2563, MAC-21: lung lymphoma: HeLa: cervical adenocarcinoma [8] CVCL_M629 ...
Proteins from both cell populations are combined and analyzed together by mass spectrometry as pairs of chemically identical peptides of different stable-isotope composition can be differentiated in a mass spectrometer owing to their mass difference. The ratio of peak intensities in the mass spectrum for such peptide pairs reflects the ...
Stable cell-cell interactions are required for cell adhesion within a tissue and controlling the shape and function of cells. [1] These stable interactions involve cell junctions which are multiprotein complexes that provide contact between neighboring cells. Cell junctions allow for the preservation and proper functioning of epithelial cell ...
In March 2020, the Cellosaurus created a page containing cell line information relevant to SARS-CoV-2 in response to the COVID-19 pandemic. [ 9 ] The Cellosaurus encyclopedia is widely recognized as an authoritative source for cell line information, providing unique identifiers [ 10 ] [ 11 ] and as source of curated information.
In cellular biology, stable cells are cells that multiply only when needed. They spend most of the time in the quiescent G 0 phase of the cell cycle but can be stimulated to enter the cell cycle when needed. Examples include the liver, the proximal tubules of the kidney and endocrine glands.
Early research (Bathurst and Jarrett, 1988) [13] found that cellular confinement reinforced gravel bases are "equivalent to about twice the thickness of unreinforced gravel bases" and that geocells performed better than single sheet reinforcement schemes (geotextiles and geogrids) and were more effective in reducing lateral spreading of infill under loading than conventional reinforced bases.
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